John Sedor Laboratory

Research

Overview

Inflammation and fibrosis - Signaling and metabolic networks

• Overall areas of interest with key words
  • Chronic kidney disease, podocyte, precision medicine, glomerulus                                             
• Tools and expertise of the lab 
  • Glomerular cell experimental cell systems (Madhavan, JCI Insight, 2017, O’Toole, J. Am Soc. Nephology, 2018, Kim, JBC, 2010, Kim, AJP Renal, 2011)
  • iPSCs
  • Transgenic mice (Bruggeman, J Am Soc Nephrol, 2016, Bruggeman, PLoSOne, 2019)
  • Spatial proteomics with APEX2 tag                          
• Examples of collaborators 
  • John O’Toole, Leslie Bruggeman – APOL1 and chronic kidney disease
  • Oliver Wessely – iPSCs kidney disease pathogenesis
  • Anant Madabhushi – computational pathology and kidney disease
  • Dana Crawford, Will Bush – registries, clinical information, gene epi                                             
• Lab discoveries
  • APOL1 expression is kidney cells mediates disease (Madhavan, JASN, 2011, JCI Insight, 2017, Bruggeman, JASN, 2016 and PlOSOne, 2019)
  • Mechanisms of APOL1 injury (Sampson, JASN, 2016, O’Toole. 2018)                                              
• How could these discoveries help patients?
  • New therapies that are mechanisms-based
  • Define kidney diseases by mechanisms rather than pathologic description
  • Predict therapy response using in vitro models derived from patient cells
  • Implementation of first precision medicine-based therapies for patients with kidney diseases

Selected Publications

View publications for John Sedor, MD
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Jayapandian, C.P., Y. Chen, A.R. Janowczyk, M. B. Palmer, C.A. Cassol, M. Sekulic, J.B. Hodgin, S.M. Hewitt, J. O’Toole, P Toro, J.R. Sedor, Barisoni, and A. Madabhushi. Development and evaluation of deep learning-based segmentation of histologic structures in the kidney cortex with multiple stains. Kidney Int. 2020 Aug 21.

Crawford, D.C., J. Lin, J.N. Cooke Bailey, T. Kinzy, J.R. Sedor, J.F. O’Toole, and W.S. Bush. Frequency of ClinVar pathogenic variants in chronic kidney disease patients surveyed for return of research results at a Cleveland public hospital. Pac. Symp. Biocomp. 25: 575-586, 2020.

Bruggeman, L.A., Z. Wu, L.Luo, S. Madhavan, P.E. Drawz, D.B. Thomas, L. Barisoni, J.F. O’Toole,and J.R. Sedor. PLoS One. 2019 Oct 29; 14: e0224408.

Madhavan, S.M., J.F. O’Toole, M. Konieczkowski, L. Barisoni, D.B. Thomas, S. Ganesan, L.A. Bruggeman, M. Buck, and J.R. Sedor.  APOL1 Variants Change C-terminal Conformational Dynamics and binding to SNARE protein VAMP8.  JCI Insight, 2017 Jul 20;2(14). pii: 92581. doi: 10.1172/jci.insight.92581.